Evidence that all messenger RNA molecules (except histone messenger RNA) contain Poly (A) sequences and that the Poly(A) has a nuclear function

The appearance of newly formed messenger RNA in polyribosomes of HeLa cells Is inhibited by over 85% by 3′deoxyadenosine (Penman, Rosbash &; Penman, 1970) probably due to the failure of normal attachment of poly(A) to heterogeneous nuclear RNA in the presence of this drug (Darnell, Philipson, Wall &; Adesnik, 1971). Results presented here show that the labeled RNA which does reach polysomes in the presence of 3′deoxyadenosine can be characterized as messenger RNA which contains smaller poly(A) segments than normal messenger RNA. The results of the present experiments suggest that all, or almost all, HeLa cell messenger RNA molecules (except for histone messenger RNA) are derived from nuclear RNA molecules which contain poly (A).     

Toxicity of Blastomyces dermatitidis

Following injections of the insoluble portion of disrupted yeast cells ofBlastomyces dermatitidis animals exhibited a biphasic pyrogenic response, a decrease in total serum proteins, and there was no release of interferon.     

The effect of exogenous hormone treatment on spermiation and vitellogenesis in the grey mullet, Mugil cephalus L.

The effects of mammalian gonadotropins, methyltestosterone and partially purified salmon gonadotropin on spermiation and oocyte maturation were studied in adult grey mullet. Methyltestosterone was a potent spermiating agent in both prespawning and spawning trials. The most effective dose was 5 mg/100 g body weight. Human chorionic gonadotropin (HCG) was moderately effective at a dose rate of 20 IU/100 g body weight. Both hormones blocked resorption of milt in captive animals. The HCG, FSH/LH combination, Synahorin+mullet pituitary homogenate, and partially purified salmon gonadotropin were equally effective, at the doses used, in inducing oocyte maturation and preventing onset of atrophy before completion of vitellogenesis. Atrophy occurred in all experimental fish at mean oocyte diameter of 750 μ. The significance of this finding is discussed. (Oceanic Institute Contribution No. 98).